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Onar Bacteria Detection Kit for PCR (12-1025)

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12-1025 25 testów 150.00 EUR netto
(184.50 EUR brutto)
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12-1050 50 testów 271.00 EUR netto
(333.30 EUR brutto)
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12-1100 100 testów 473.00 EUR netto
(581.80 EUR brutto)
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12-1250 250 testów 1008.00 EUR netto
(1240.00 EUR brutto)
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Onar Bacteria Detection Kit for conventional PCR

Kit for in vitro diagnosis of eubacteria

 

The first PCR assay called OnarBacteria Detection Kit has been developed for accurate and reliable in vitro diagnosis of bacteria. The kit provides laboratories with a simple and rapid means to confirm eubacteria infection in various in situ biologicals including cell cultures.

 

Validate Your Experimental Results

Frequent screening of cell cultures for eubacteria and mycoplasma contamination is critical for reliable results. In addition to the Eubacteria Detection Kit, Minerva Biolabs offers detection kits for mycoplasma and viruses for conventional and real-time applications.

 

Benefits of OnarBacteria Detection Kit:

- Sensitive
Detection with fewer than 12 (e.g. Bacillus subtilis) genomes per sample volume.

- Specific
Detection of more than 45 eubacteria species: Actinomyces, Bacillus, Enterococcus, Escherichia coli, Fusobacterium, Klebsiella, Lactobacillus, Micrococcus, Mycobacterium, Peptostreptococcus, Pseudomonas, Porphyromonas, Prevotella, Salmonella, Serratia, Staphylococcus, Streptococcus, etc.

- Reliable
The kit contains positive control DNA, and an internal control to eliminate the possibility of false-negative results.

- Rapid
The whole procedure can be completed in about 3.5 hours. Hands-on time less than 40 min.

- Convenient
The reagents are pre-aliquoted for 25 reactions for convenient periodic or routine testing.

- Ease of use
Easy analysis by comparison with the positive control.

 

Test Principle

OnarBacteria Detection Kit utilizes the polymerase chain reaction (PCR), thereby providing the highest sensitivity for the detection of eubacteria contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 12 eubacteria genomes per sample volume. The primer set is specific to the 16S ribosomal RNA coding region in the eubacteriae genome. This allows for detection of all eubacteria species tested thus far and usually encountered as airborne contaminants in cell cultures. Eukaryotic DNA is not amplified by OnarBacteria Detection Kit. Only one protocol is needed for the detection of eubacteria species, and can be completed within 3.5 hours. Onar Bacteria also provides internal control DNA to assure successfully executed reactions. OnarBacteria Detection Kit should be performed only with EUB -Polymerase. In principle the kit can be perfomed with any polymerase. However, polymerases of different manufacturers can be contaminated with bacterial DNA leading to false-positive results. Therefore we strictly recommend the use of only DNA-free polymerase (Cat. No.: 54-0100).

 

Background

Bacteria Contamination is Common.

Bacteria are frequently found as contaminants in cell cultures. Studies have identified an overall 6.5% incidence of static bacterial contamination of cell cultures examined. Thus, many cell cultures lack visual signs of bacterial contamination, generally indicated by decoloration of the fluid. Moreover, it has been demonstrated that standard antibiotics not only are uneffective against resistant baterial infection but also have a strong impact on the metabolism, cell growth & differentiation.

 

Recommended Use / Scope

Applicable in research and industry for direct testing of cell cultures and biologicals. For QA application specific validation might be required. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

 

Kit Components

- Lyophilized Reaction Mix including the Internal Amplification Control and 10x reaction buffer optimized for DNA-free EUB Polymerase

- Lyophilized positive control DNA

- PCR grade Water

 

Result evaluation

gel electrophoresis at endpoint of PCR

 

Required Consumables

- DNA-free polymerase. We highly recommend our reliable EUB Polymerase, Cat.-No. 54-0100.

PCR reaction tubes

 

Required lab devices

- Regular PCR cycler

- Agarose gel electrophoresis and DNA staining system

- Pipetting equipment

- Tube centrifuge

 

Shelf Life and Storage

Components are maintainable at +2 to +8 °C for at least 6 months. After rehydratisation the reagents must be stored at -18 °C.

 


Producent: MINERVA BIOLABS

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