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VenorGeM OneStep - Mycoplasma Detection Kit (11-8025)

Nr. kat Wielkość Cena Dodaj do koszyka
11-8025 25 testów 146.00 EUR netto
(179.60 EUR brutto)
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11-8050 50 testów 268.00 EUR netto
(329.60 EUR brutto)
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11-8100 100 testów 470.00 EUR netto
(578.10 EUR brutto)
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11-8250 250 testów 1000.00 EUR netto
(1230.00 EUR brutto)
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Venor®GeM and Venor®GeM-OneStep employ PCR technology for rapid and reliable detection of mycoplasma in various in situ biologicals including cell cultures and virus stocks. For fast, reliable and time-saving routine monitoring!

 

The Venor®GeM-OneStep is a complete kit for qualitative direct detection of mycoplasma contaminations in biologicals like cell cultures, sample materials and other culture media. The Kit includes all reagents required for PCR. Primer, nucleotides, polymerase and the internal amplification control are provided ready-to-use  in a lyophylized reaction mix. Just add the included rehydration buffer to the mix, aliquot and add sample or Positive Control DNA and you are ready in one work step.

 

Benefits of Venor®GeM-OneStep

 

- Convenient
The Venor®GeM-OneStep Detection Kit includes the polymerase and the internal amplification control in the lyophylized reaction mix

- Highly sensitive 
Detection of 1,5 copies/µl of mycoplasma DNA that corresponds to 3 mycoplasma per sample volume. LOD95% = 4,5 copies/µl.

- Specific 
Venor®GeM is specific for more than 79 mycoplasma species.

- Reliable 
The kit contains internal control DNA and positive control DNA to optimize reproducibility and minimize operator-dependent variability of results.

- Validated 
Venor®GeM was intensively validated according to the guidelines of the European Pharmacopoeia, section 2.6.7.

- Simple
Templates for PCR analysis are prepared by boiling the supernatant of cell cultures or other sample material.

- Clear
A clear and easily interpretable result is obtained with one PCR reaction.

 

Test Principle

 

Minerva Biolabs Mycoplasma Detection Kit Venor®GeM and Venor®GeM-OneStep incorporate the highly-sensitive PCR technology as its method for detecting contaminating mycoplasma, which also offers outstanding characteristics over other techniques and products used for mycoplasma safety.

 

Screen frequently to validate your experimental results

 

Screening of cell cultures at regular intervals for mycoplasma contamination is critical for reliable and reproducible results. We recommend to screen cell cultures on a monthly basis as well as before cryopreservation. In addition to our Venor®GeM and Venor®GeM-OneStep Mycoplasma Detection Kits, we offer a mycoplasma screening service.

 

Quality Control Considerations

 

Mycoplasma are often found as common contaminants in cell cultures, virus stocks, and other cell-derived biologicals. Mycoplasma contamination is a recurring problem that can cause non-reproducible results in research and productivity losses of up to 50 % in industry, which can be avoided by routine diagnostic testing. Nowadays, testing for mycoplasma infection is an essential requirement in reaching quali-ty control standards for all cell culture laboratories. It is necessary to demonstrate quality in laboratories concerned with the production and maintenance of cell lines used in manufacturing various biologicals and in research laboratories to validate their results.

 

Positive and Internal Controls

 

Venor®GeM and Venor®GeM-OneStep incorporate the highly-sensitive PCR technology as its method for detecting contaminating mycoplasma, which also offers outstanding characteristics over other techniques and products used for mycoplasma safety. The kit includes positive and internal controls. The internal control confirms proper amplification by acting as an alternative template for the PCR primers. Non-infectious Mycoplasma orale DNA is used as a positive control.

 

Background

 

Mycoplasma are known to cause a variety of human and animal diseases, and in the laboratory, mycoplasma are often found as common contaminants in cell cultures, virus stocks, and other cell-derived biologicals. Mycoplasma contamination is a recurring problem that can cause non-reproducible results in research and productivity losses of up to 50% in industry, which can be avoided by routine diagnostic testing. Also, testing for mycoplasma infection is now an essential requirement in reaching quality control standards for laboratories concerned with the production and maintenance of cell lines used in manufacturing various biologicals.

 

Type of PCR

 

for conventional, endpoint PCR

 

Recommended Use / Scope

 

Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

 

Kit Components


- primer sets, nucleotides, internal amplification control and polymerase at optimized concentrations - lyophilized in one tube

- rehydration buffer

- lyophilized positive control

- PCR grade water

 

Result evaluation

 

gel electrophoresis at endpoint of PCR

 

Required Consumables

 

- agarose gels and DNA stains

- PCR reaction tubes

 

Required lab devices

 

- gel electrophoresis and documentation system

- regular PCR cycler

- pipetting equipment

- tube centrifuge

 

Shelf Life and Storage

 

Venor®GeM-OneStep components are maintainable at +2 to +8 °C for at least 6 months. After rehydratisation the reagents must be stored at -18 °C.

 

EP 2.6.7 compliance

 

Use of Venor®GeM-OneStep for QA testing of biologicals like master and working cell banks, autologous cells, culture media, bulk harvest and final product testing according to EP 2.6.7 might be applicable after appropriate sample preparation and process validation. Please contact our service line for assistance.

 


Producent: MINERVA BIOLABS

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