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kulki M-pluriBeads

M-Beads - UNIVERSAL (31-XXXXX-20)

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31-XXXXX-20 2 ml 126.00 EUR netto
(155.00 EUR brutto)
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pluriBead® is a unique cell separation technology that works without any magnetic components. The procedure is simple: You sieve your pluriBead (with bound target cells) down a strainer - the pluriBeads with your target cells stay on top, the unwanted cells run through. After detaching, you have your target cells ready.

 

pluriBead suspension contains non-magnetic monodisperse microparticles (beads) for the sorting of cell mixtures. The surface of the beads is modified with antibodies that recognize specific structures on the cell surface. The beads are larger than the cells and enlarge these artificially. This enlargement of the cells allows a simple separation through strainers as well as the use of different separation strategies. 

 

Universal beads are developed for use with any external antibody. They carry monoclonal and polyclonal antibodies produced from the serum of a host animal and are available for 5 different species. pluriBead universal is a non-magnetic cell separation method that will catch up to 3*10^7 targets per separation. Since the beads can be used for working at room temperature (or even 37°C) under physiological conditions, cell stress is kept very low. Time consuming sample preparation is not required - the beads can be mixed directly with all biological solutions.

 

Universal pluriBead allows for the easy designing of customized catcher particles to bind and isolate the required targets.

Applications:
- You have a specific antibody but no technology to apply it to.
- You need a specific antibody and want to test product performances.

We offer Universal pluriBeads for following species:
- Mouse
- Rat
- Hamster
- Goat
- Rabbit

Application procedure for pluriBead Universal:
The coupling of universal beads with any external antibody (min. 10 µg) is done in 3 easy steps.

 

1) Mixing of pluriBead Universal with your antibody and PBS solution Mixing of pluriBead Universal with your antibody and PBS solution.
2) Incubation of the mixture for 3-4 hrs. at room temperature or 4°C Incubation of the mixture for 3-4 hrs. at room temperature or 4°C.
3) Washing of the custom labeled beads Washing of the custom labeled beads.

After labeling, the beads can be used according to our standard protocols (whole blood, buffy coat, tissue). 

 

S-pluriBeads:
Size: 30 µm    
Maximum Target Cells Per Separation: 10^7    
pluriBead Suspension: 2 ml
Maximum Bead Suspension Per pluriStrainer: 400 µl    
Sample Material: Whole Blood, Tissue, PBMC, Cell Culture    
Recommended Application: small number of targets in large sample volume and Circulating Tumor Cells (CTC)
Minimum Sample Volume: 200 µl

M-pluriBeads:
Size: 60 µm
Maximum Target Cells Per Separation: 5*10^7
pluriBead Suspension: 2 ml
Maximum Bead Suspension Per pluriStrainer: 1000 µl
Sample Material: Buffy Coat, Whole Blood, Tissue, PBMC, Cell Culture
Recommended Application: large number of targets in a samples (e. g. Buffy Coat)
Minimum Sample Volume: 500 µl

 

VIDEO TUTORIALS:

Video Tutorial - pluriStrainer:

https://www.youtube.com/watch?v=7pKtNi1Y428

Cell Separation from whole blood:

https://www.youtube.com/watch?v=fZR1dyQoZbw

pluriBead - simple cell isolation directly from whole blood:

https://www.youtube.com/watch?v=VXEUof7hHkU

Minimize artifacts of RNA expression and protein profiling in less then 10 min:

https://www.youtube.com/watch?v=dsBP3HL-CNQ

pluriBead Cascade (Multi Target Cell Separation):

https://www.youtube.com/watch?v=XHM7nCJnV0M

 

SAMPLE MATERIAL
You can use our pluriBead Kits on whole blood, cell culture, PBMC and buffy coat. Keep in mind that each sample material needs different treatment.  

AVAILABLE PRODUCTS:

31-MSaHU-20 universal M-pluriBead® anti-hu pluriBead® universal are developed for the use with external antibodies. They are coated with a polyclonal antibody produced from the serum of a host animal.  (2ml)
31-GTaMS-20 universal M-pluriBead® anti-ms pluriBead® universal are developed for the use with external antibodies. They are coated with a polyclonal antibody produced from the serum of a host animal.  (2ml)
31-GTaRT-20 universal M-pluriBead® anti-rat pluriBead® universal are developed for the use with external antibodies. They are coated with a polyclonal antibody produced from the serum of a host animal.  (2ml)
31-GTaRB-20 universal M-pluriBead® anti-rabbit pluriBead® universal are developed for the use with external antibodies. They are coated with a polyclonal antibody produced from the serum of a host animal. (2ml)
31-GTaHS-20 universal M-pluriBead® anti-hamster pluriBead® universal are developed for the use with external antibodies. They are coated with a polyclonal antibody produced from the serum of a host animal. (2ml)
31-RBaGT-20 universal M-pluriBead® anti-goat pluriBead® universal are developed for the use with external antibodies. They are coated with a polyclonal antibody produced from the serum of a host animal. (2ml)


     
     
     
     
     


Producent: PLURISELECT

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